SIV mac251直腸途徑感染獼猴模型

更新時(shí)間：2026-05-16 來(lái)源：健明迪檢測(cè)

健明迪檢測(cè)提供的SIV mac251直腸途徑感染獼猴模型，討論與結(jié)論 1.該模型制備比較成熟，國(guó)內(nèi)外都有此模型，構(gòu)建和評(píng)價(jià)指標(biāo)一致，所以該模型應(yīng)用廣泛，具有統(tǒng)一的操作規(guī)程，具有CMA，CNAS認(rèn)證資質(zhì)。

討論與結(jié)論

1.該模型制備比較成熟，國(guó)內(nèi)外都有此模型，構(gòu)建和評(píng)價(jià)指標(biāo)一致，所以該模型應(yīng)用廣泛，具有統(tǒng)一的操作規(guī)程。

2. 該模型制備的關(guān)鍵：1）小鼠必須是重度免疫缺陷的，而且飼養(yǎng)環(huán)境必須潔凈；2）HIV操作需要生物安全。

3.此模型具有完善的人的免疫系統(tǒng)，HIV感染后，具有艾滋病病人相似的臨床癥狀，所以具有很高的比較醫(yī)學(xué)應(yīng)用價(jià)值。

生物安全性

所有感染動(dòng)物必須在ABSL-3室中進(jìn)行。

評(píng)價(jià)驗(yàn)證

診斷：病毒在血液中復(fù)制。

制備方法

Three vaccines were tested in groups of 8 MCM with each animal being vaccinated intradermally or intramuscularly with 100μg DNA primes at weeks 0, 4 and 8 and a rAd5 boost (6x1010 particles) at week 16.Eight unvaccinated animals served as controls for each group challenged with the same virus stock.The IM vaccination experiments were performed subsequent to the ID vaccinations and employed an additional group of 8 control animals that were challenged in parallel. From week 23 the animals were challenged intrarectally with 150TCID50 of SIVmac251 at weekly intervals for up to 10 weeks or until they became infected, as determined by virus in the plasma.

研究背景

來(lái)源文獻(xiàn)：Vaccination of macaques with DNA followed by adenoviral vectors encoding SIV gag alone delays infection by repeated mucosal challenge with SIV.

PMID：31413132

DOI：10.1128/JVI.00606-19